Complete genome sequence of actinobaculum schaalii strain. Pdf in vitro susceptibility of actinobaculum schaalii to. Actinobaculum schaalii is a facultative anaerobic, grampositive rodshaped species phylogenetically related to actinomyces that is likely part of the commensal flora of the human genitourinary tract. It grows slowly and therefore is easily overgrown by other pathogens, which are often found concomitantly. Actinobaculum schaalii is a grampositive, facultative anaerobic coccoid rod, classified as a new genus in 1997. To assess the in vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents as well as to dissect the genetic basis of. Two newly described species, actinobaculum massiliae and actinobaculum urinale, were recovered from the urine of elderly women with chronic cystitis 6, 7. Actinobaculum schaalii can cause urinary tract infec tions and. Objectives to assess the in vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents as well as to dissect the genetic basis of fluoroquinolone resistance. In vitro susceptibility of actinobaculum schaalii to 12. Jan 01, 2014 actinobaculum schaalii is an emerging uropathogen. Cattoir v, varca a, greub g, prodhom g, legrand p, et al.
The aim of this study was to determine the in vitro susceptibility of 48 a. Dec 17, 2015 actinobaculum schaalii is an emerging pathogen particularly involved in urinary tract infection of elderly people andor patient with urological risk factors of urinary tract infection. We report 12 patients with actinobaculum bacteremia. Actinotignum schaalii formerly actinobaculum schaalii. Since 1999, actinobaculum schaalii is routinely investigated at our hospital, whenever its presence is suspected due to the detection of minute grey colonies on. The molecular analysis of fluoroquinolone resistance was also attempted by determining the qrdr sequences of the gyra and parc genes. Cattoir v, varca a, greub g, prodhom g, legrand p, lienhard r. This bacterium was classified as a new genus in 1997 but is often overlooked or considered a contaminant because of both its resemblance to the normal bacterial flora on skin and mucosa and the overgrowth of other bacteria. Actinobaculum schaalii an emerging pediatric pathogen. Cattoir v, varaca a, greub g, prodhom g, legrand p, lienhard r. It is a grampositive, facultative anaerobic coccoid rod, considered a human pathogen. Only 40% of blood cultures were clinically considered significant by the treating physicians, but most patients were treated for uti, suggesting a possible urinary source of bacteremia. Comparison of isolated actinobaculum schaalii with related human pathogens reported in the literature characteristic isolate that caused endocarditis reaction of reference a.
Actinobaculum schaalii is a grampositive coccoid rod that causes various infections in humans and is easily overlooked in cultures. Clinical and microbiological features of actinotignum bacteremia. Clinical and microbiological features of actinotignum. All were susceptible to penicillin mic, metronidazole mic, 256. Actinobaculum species are anaerobic grampositive rods that have previously been associated with urinary tract infection uti in the elderly. Its subdisciplines are virology study of viruses, bacteriology study of bacteria. We report urosepsis with actinobaculum schaalii detected serendipitously in blood and urine culture in a 79yearold with urinary tract obstruction. Antimicrobial activity antibacterial activity introduction the science dealing with the study of the prevention and treatment of diseases caused by microorganisms is known as medical microbiology. In vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents and molecular analysis of fluoroquinolone. Nov 11, 2015 the genus actinotignum contains three species, actinotignum schaalii formerly actinobaculum schaalii, actinotignum urinale and actinotignum sanguinis. Peymantavassoli,1 ryanpaterson,2 andjennifergrant1 1department of pathology and laboratory medicine, university of british columbia, vancouver, bc, canada v6t 2b5 2department of urologic sciences, university of british columbia, vancouver, bc, canada v5z 1m9. In vitro susceptibility of actinobaculum schaalii to 12 antimicrobial. So far, its identification has been performed with 16s rrna gene sequencing or pcr.
In vitro susceptibility of actinobaculum schaalii to. Actinobaculum schaalii causing epididymitis in an elderly. Background actinobaculum schaalii was first described as a causative agent for human infection in 1997. To assess the in vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents as well as to dissect the genetic basis of fluoroquinolone resistance.
Actinotignum schaalii formerly known as actinobaculum schaalii is a bacterium first isolated from human blood cultures. Since then it has mainly been reported causing urinary tract infections uti in elderly individuals with underlying urological diseases. Actinobaculum schaalii was first described as a causative agent for human infection in 1997. Each isolate was identified by 16s rrna sequencing.
Routine urine samples were obtained from 177 hospitalized patients and 75 outpatients in viborg county, denmark, in 20082009. To assess the in vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents as. Oct 26, 2011 actinobaculum schaalii is a grampositive, facultative anaerobic coccoid rod, classified as a new genus in 1997. In vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents and molecular analysis of. In vitro activity of 12 antimicrobial agents against 48 clinical isolates of a. Infections related to actinotignum schaalii formerly. Actinobaculum schaalii is a grampositive facultative. Pdf in vitro susceptibility of actinobaculum schaalii to 12. In vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents and molecular analysis of fluoroquinolone resistance. Schaal, contemporary german microbiologist, for his contributions to actinomycete microbiology. Here, we report the first case of prosthetic joint infection due to a.
Gram stain of actinobaculum schaalii, grown in anaerobic blood culture bottle. This paper illuminates the flaws in our current system in detecting a. Actinobaculum schaalii can cause urinary tract infections and septicemia but is difficult to identify by cultivation. Actinobaculum schaalii, a common uropathogen in elderly. Actinotignum schaalii formerly actinobaculum schaalii is a facultative anaerobic grampositive rodshaped bacterium considered as a commensal bacterium of the genital or urinary tract 1,2. Apr 09, 2012 actinobaculum schaalii is a small grampositive coccoid rod that requires co 2 for optimal growth. The bacterium is considered to be a part of the normal flora in the genital and urinary tract area 2. Since 1999, actinobaculum schaalii is routinely investigated at our hospital, whenever its presence is suspected due to the detection of minute grey colonies on blood agar. Actinobaculum images partners infectious disease images.
Oct 01, 2014 read recommendations for optimal detection and identification of actinobaculum schaalii in urine, apmis on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Actinobaculum schaalii was first described as a causative agent for. Isolation and identification is challenging and often needs molecular techniques. Actinobaculum schaalii, a commensal of the urogenital area. Lienhardin vitro susceptibility of actinobaculum schaalii to 12 antimicrobial agents and molecular analysis of fluoroquinolone resistance. Complete genome sequencing of the emerging uropathogen actinobaculum schaalii indicates that an important mechanism of its virulence is attachment pili, which allow the organism to adhere to the surface of animal cells, greatly enhancing the ability of this organism to colonize the urinary tract. Actinobaculum schaalii invasive pathogen or innocent. However, it is underestimated as a cause of urinary tract infections utis because of its slow growth on common media and the difficulty of identification by standard phenotypic methods.
Nine of 10 strains of actinobaculum schaalii caused urinary tract infections in predisposed. Actinobaculum schaalii, a new cause of knee prosthetic joint. Ermxmediated resistance to macrolides, lincosamides and streptogramins in actinobac ulum schaalii article pdf available in journal of antimicrobial chemotherapy 698 april 2014 with 80 reads. Since then it has mainly been reported causing urinary tract infections uti in elderly. This microorganism is a difficult to diagnose pathogen and is rarely involved in systemic or deep infections. In vitro activity of antimicrobial agents against actinotignum schaalii clinical isolates. The genus actinotignum contains three species, actinotignum schaalii formerly actinobaculum schaalii, actinotignum urinale and actinotignum sanguinis. The diagnosis has often been delayed due to fastidious growth and identification problems.
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